Composite

Part:BBa_K273011

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)

PLlac - RBS - kivd - RBS - adh2(Y) - Ter

This construct produces butanol and further higher branched-chain alcohols in a non-fermentative manner exploiting the hosts amino acid synthesis intermediates.

We used this construct for testing and characterization purposes in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None